Agencourt RNAClean XP

The Agencourt RNAClean XP kit provides a simple, flexible and highly reproducible method for purifying nucleic acid products generated in common enzymatic reactions such as cDNA synthesis and in vitro transcription (IVT) reactions.

Application: Post-reaction cDNA and cRNA cleanup

Downstream Application Techniques: Gene expression via microarray analysis (using Eberwine RNA amplification protocol^‡)

Agencourt RNAClean XP Features

• Purification of small and large nucleic acid products
• Complete removal of salts, unincorporated primers and dNTPs
• Simple automation-friendly protocol
• No centrifugation, filtration or precipitation steps required
• Elution in aqueous solution
• Purifies both cDNA and cRNA
• Scalable throughput

Process Overview

1. Enzymatic reaction 2. Binding of cDNA to magnetic beads 3. Separation of total RNA bound to magnetic beads from contaminants 4. Washing of RNA with Ethanol 5. Elution of RNA from the magnetic particles
6. Transfer away from the beads into a new plate

High Yield

The Agencourt RNAClean system consistently recovers more cRNA than standard column-based cleanup methods. The kit is effective in two methods for producing cRNA for microarray analysis, IVT and NuGEN* Amplification Technology. As shown in Figure 1, the Agencourt RNAClean XP kit produced a higher yield of RNA over the RNeasy* kit across both amplification methods.

Figure 1. In vitro transcribed cRNA products produced from the same biological sample were purified using the Agencourt RNAClean XP kit or the RNeasy column-based system. IVT target was PCR products that contained a T7 sequence on the forward primer. IVT was performed using an Ambion* MEGAscript* T7 Kit. NuGEN Target was total RNA from mouse liver extracted using the Agencourt RNAdvance Tissue kit. Amplification was performed using the NuGEN RNA amplification system V2. Samples were pooled and diluted prior to extractions. Chip analysis was performed using RNA Nano Chip on the Agilent* Bioanalyzer.

Versatile and Economical

The Agencourt RNAClean XP kit is a fast and flexible solution for cleaning up enzymatic reactions. To accommodate various existing laboratory processes, the RNA purified using this chemistry can be run using a variety of IVT labeling kits. Even without the use of organic solvents, centrifugation, or vacuum filtration, RNA is purified in around 20 minutes.

Ordering Information

To request a quote or make an inquiry use the on-line contact form.
In the United States and Canada call +1 800 361 7780.
Outside the United States and Canada consult the International Distributors list.