Agencourt Genfind v2

The Agencourt Genfind v2 DNA purification system isolates and purifies genomic DNA (gDNA) from whole blood, serum and FTA cards. The kit is powered by cutting-edge Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology to effectively produce a high recovery of DNA for downstream applications such as PCR^† and genotyping. SPRI allows for fast separation, easy manipulation and simple automation compared to traditional centrifugation and vacuum filtration technologies. The method can be run manually in a 2 mL tube format or automated format on the Beckman Coulter Biomek NX^P or FX^P workstations.

Application: Genomic DNA isolation from whole blood, plasma, serum, and FTA cards. Additional protocols are available for extracting genomic DNA from cultured eukaryotic cells, bacteria, and fresh or frozen tissue. Please inquire with your sales representative for these additional protocols.

Downstream Application Techniques: Genotyping applications (SNP, fragment analysis), clinical sequencing, resequencing, qPCR

Agencourt Genfind v2 Features

Whole blood processing from 50 to 400 µL volumes
High gDNA recovery up to 6 µg from 200 µL of whole blood
No centrifugation or vacuum filtration required
Biomek Span-8 and 96-Multichannel methods are available for whole blood
Automated extraction of 96 samples in approximately 2.5 hours

Process Overview

1. Lyse whole blood or serum in Lysis Buffer and Proteinase K 2. Bind genomic DNA to paramagnetic beads 3. Separate beads from contaminants 4. Wash the magnetic beads with Wash Buffer 1 to remove contaminants 5. Wash the magnetic beads with Wash Buffer 2 to remove contaminants 6. Elute DNA from magnetic particles 7. Transfer to new plate

Consistent and High Recovery of gDNA

Figure 1 demonstrates that Agencourt Genfind v2 delivers high quality and consistent yields in comparison to competitor filtration and bead-based methods. The unique properties of the SPRI technology allows for the effective capture of gDNA in the presence of various anticoagulants.

Figure 1. Replicates of 8 200 µL samples of frozen human blood were manually extracted and eluted in 200 µL H2O (High Pure was eluted in 10 mM tris, pH 8.5); for all kits analyzed, aliquots were taken from the same patient sample for each anticoagulant used. DNA concentrations and yields were determined using the Beckman Coulter DTX plate reader to measure the A260 absorbance.

As seen in Table 1, Ct values obtained in qPCR reactions indicate that samples purified by the Agencourt Genfind v2 system produce amplifiable gDNA with a high sample-to- sample consistency.

Comparison of Ct Values

Sample	Mean Ct
Agencourt Genfind v2 10 ng	21.50 ± 0.37
MagneSil Blood Genomic Kit 10 ng	21.40 ± 0.15
QIAamp Blood Mini Kit 10 ng	20.18 ± 0.20
High Pure PCR Template Preparation Kit 10 ng	21.35 ± 1.03
Agencourt Genfind v2 1 ng	24.19 ± 0.17
MagneSil Blood Genomic Kit 1 ng	24.23 ± 0.48
QIAamp Blood Mini Kit 1 ng	22.96 ± 0.31
High Pure PCR Template Preparation Kit 1 ng	24.35 ± 0.77

Comparison of Ct values for nucleic acid purified using the Agencourt Genfind v2 system, MagneSil* Blood Genomic kit, QIAamp* Blood Mini kit, and High Pure* PCR Template Preparation kit. Indicated amounts of DNA manually extracted from frozen human blood prepared with EDTA were used in the qPCR analysis.

Figure 2. Gel electrophoresis of 1 µL genomic DNA through a 0.8% agarose gel. DNA was isolated from 200 µL blood with the Agencourt Genfind v2 purification method and eluted in 200 µL H2O. Key: M = 1 kb ladder, lanes 1 & 2 = Citrate, lanes 3 & 4 = EDTA, and lanes 5 & 6 = Heparin.

High Quality gDNA

The Agencourt Genfind v2 DNA isolation method isolates and purifies gDNA that is intact and suitable for downstream PCR and genotyping. Genomic DNA from human whole blood samples was isolated with Agencourt Genfind v2 and analyzed by gel electrophoresis (Figure 2).

Summary

The Agencourt Genfind v2 system is efficient and automation friendly. By automating the process using Agencourt software methods on either Biomek Span-8 or 96 Multichannel Biomek workstation, gDNA can be isolated and purified in approximately 2.5 hours. With the power of SPRI chemistry, the Agencourt Genfind v2 system produces highly pure DNA and consistent data thus allowing researchers to minimize retesting of precious samples.

Complete Automation

The use of the Agencourt SPRI paramagnetic bead-based technology allows Agencourt Genfind to be fully automated while maintaining a high sample quality. Genfind is currently optimized on Beckman Coulter’s Biomek FX, FX^P, NX, and NX^P Laboratory Automation Workstations using either a 96-well multichannel head or a Span-8 head.

Ordering Information

To request a quote or make an inquiry use the on-line contact form.
In the United States and Canada call +1 800 361 7780.
Outside the United States and Canada consult the International Distributors list.

Product	Size	Kit Components	Product #
Agencourt Genfind v2 Tube Kit	50 preps	Lysis Buffer Wash I Buffer Wash II Buffer Binding Buffer Proteinase K Proteinase K Buffer	A41499
Agencourt Genfind v2 96-well Plate Kit	384 preps (4 x 96)	Lysis Buffer Wash I Buffer Wash II Buffer Binding Buffer Proteinase K Proteinase K Buffer	A41497

Related Products			Product #
Software - Agencourt Genfind v2 96 MC - 3.x			A42568
Software - Agencourt Genfind v2 96 - Biomek Span-8 - 3.x			A42569
Software - Agencourt Genfind v2 FTA Cards 96 MC - v 3.x			A46822
Agencourt AMPure PCR Purification Kit (60 ml)			A29152
Agencourt SPRIStand - Magnetic 6-tube Stand			A29182
Agencourt SPRIPlate Super Magnet Plate			A32782

† The PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffman-La Roche, Ltd.
* All trademarks are property of their respective owners.
Beckman Coulter, the stylized logo, Agencourt, AMPure, Biomek, Genfind, SPRI, SPRIPlate, and SPRIStand are registered trademarks of Beckman Coulter, Inc.