Agencourt DNAdvance

Built on Solid Phase Reversible Immobilization (SPRI) paramagnetic bead-based technology, the Agencourt DNAdvance kit is a high throughput genomic DNA (gDNA) isolation reagent kit enabling the purification of high quality DNA from mammalian tissue samples. The Agencourt DNAdvance kit offers greater recovery of high quality gDNA than other commercially available DNA isolation kits.

Application: Nucleic acid extraction from mammalian tissue samples

Downstream Application Techniques: Genotyping applications (SNP, fragment analysis), clinical sequencing, resequencing, qPCR^†

Agencourt DNAdvance Features

• Able to process three 96-well plates in about 75 minutes when using the Beckman Coulter Biomek NX^P or FX^P 96 Multichannel laboratory automation workstations.
• No centrifugation or vacuum filtration required
• Compatible with a variety of downstream analysis tools such as PCR, qPCR, SNP genotyping, and sequencing
• Does not use organic extraction or centrifugation/filtration

Process Overview

1. Lyse up to 20 mg specimen in Lysis Buffer, DTT, and Proteinase K 2. Bind genomic DNA to paramagnetic beads 3. Separate beads from contaminants 4. Wash the magnetic beads with 70% Ethanol to remove contaminants 5. Elute DNA from magnetic particles 6. Transfer to new plate.

Higher Recovery of Nucleic Acid

By using SPRI technology to isolate nucleic acid from mammalian tissue, Agencourt DNAdvance consistently delivers superior recovery of nucleic acid. Figure 1 shows yields obtained from 20 mg rodent tail samples with DNeasy*, Wizard* Magnetic 96, and Agencourt DNAdvance.

Figure 1. Nucleic acid yields from 24 replicates of 20 mg rodent tail samples. Yields determined spectrophotometrically.

As seen in Figure 2, the Agencourt DNAdvance kit produces nucleic acid of comparable quality in comparison to DNeasy. In order to appear on the gel, Wizard Magnetic 96 required four times the material.

Figure 2. Results from 1 µl of eluent from Agencourt DNAdvance (A) 1 µl of eluent from 100 µl using DNeasy (B) and 4 µl of eluent from 500 µl using Wizard Magnetic 96 (C). Extractions were electrophoresed on an 0.8% agarose gel. Wells 1-8 contain samples, Well M contains the 1kb ladder.

Nucleic Acid from Different Sample Types

The Agencourt DNAdvance kit is versatile and can extract nucleic acid from many sample types. As seen in Figure 3, it can extract nucleic acid from rat brain and rat liver samples as well as mouse tail.

Figure 3. Nucleic acid yields from 24 replicates of 20 mg samples of rat brain, rat liver and mouse tail. Yields determined spectrophotometrically.

Summary

The Agencourt DNAdvance isolation kit is an efficient, automation friendly solution for extracting nucleic acid from mammalian tissue samples. When used with the Beckman Coulter Biomek NX^P or FX^P Multichannel 96 laboratory automation workstation and Agencourt software methods, this system is simple to use and yields superior results. Supported by the power of SPRI chemistry, the Agencourt DNAdvance system produces more nucleic acid over competitive methods without PCR inhibition.

Ordering Information

To request a quote or make an inquiry use the on-line contact form.
In the United States and Canada call +1 800 361 7780.
Outside the United States and Canada consult the International Distributors list.