Agencourt AMPure XP

The Agencourt AMPure XP system is a highly efficient, easily automated PCR^† purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats.

Application: PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

Agencourt AMPure XP Features

High recovery of amplicons, greater than 100 bp
Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
Efficient recovery of double stranded and single stranded DNA templates
Consistent recovery throughout the kit’s 12 month shelf life
Faster manual and automated processing as compared to traditional post-PCR cleanup methods

Process Overview

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

High Recovery

Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

Walk-away Automated Solution

Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NX^P and FX^P for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.

Ordering Information

To request a quote or make an inquiry use the on-line contact form.
In the United States and Canada call +1 800 361 7780.
Outside the United States and Canada consult the International Distributors list.

Product	Size	Kit Components	Product #
Agencourt AMPure XP 5 mL Kit	139/278 preps¹	Agencourt AMPure XP Reagent	A63880
Agencourt AMPure XP 60 mL Kit	1,666/3,333 preps¹	Agencourt AMPure XP Reagent	A63881
Agencourt AMPure XP 450 mL Kit	12,500/25,000 preps¹	Agencourt AMPure XP Reagent	A63882

Related Products			Product #
Agencourt SPRIPlate Super Magnet Plate			A32782
Agencourt SPRIPlate 384 Magnet Plate			A29165

1 Typical reaction volume is 20 µL for the 96-well microplate and 10 µL for the 384-well microplate.

† The PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffman-La Roche, Ltd.
* All trademarks are property of their respective owners.
Beckman Coulter, the stylized logo, Agencourt, AMPure, Biomek, CleanSEQ, Genfind, SPRI, and SPRIPlate are registered trademarks of Beckman Coulter, Inc.