Agencourt AMPure Publications

• A Versatile Ligation-Independent Cloning Method Suitable For High-Throughput Expression Screening Applications NS Berrow, D Alderton, S Sainsbury, J Nettleship - Nucleic Acids Research, 2007 - Oxford Univ Press ... All other PCR products were purified using AMPure magnetic beads (Beckman-Coulter, High Wycombe, Buckinghamshire, UK) according to the manufacturer's ...
• High-Throughput Species Identification: From DNA Isolation To Bioinformatics DE RICHARDSON, JD VANWYE, AMYM EXUM, RK COWEN, DL - Molecular Ecology Notes, 2007 - ingentaconnect.com ... PCR products were then purified using the standard AmPure kit (Agencourt) protocol on the liquid- handling robot, but with a 1:1 ratio of magnetic beads to PCR ...
• The Genetic Spectrum Of A Population-Based Sample Of Familial Hemiplegic Migraine LL Thomsen, M Kirchmann, A Bjornsson, H Stefansson - Brain, 2007 - Oxford Univ Press ... TM . PCR products were verified for correct length on an agarose gel before being purified using AMPure TM from Agencourt. Cycle ...
• DNA Barcoding Using Chitons (Genus Mopalia) RP KELLY, IN SARKAR, DJ EERNISSE, ROB DESALLE - Molecular Ecology Notes, 2007 - ingentaconnect.com ... PCR products were cleaned using either 96-well filter plates (Millipore Corp.) or AMPure beads (Agencourt Corp., protocols available from manufacturer). ...
• Casting the Percomorph Net Widely: The Importance of Broad Taxonomic Sampling in the Search for the Placement of Serranid and Percid Fishes WL Smith, MT Craig, JM Quattro - Copeia - bioone.org ... were desalted and concentrated using an ArrayIt PCR Product Purification ... modifications to the manufacturer's protocol or using AMPure (Agencourt Biosciences)...
• Epidermal Growth Factor Receptor Mutation Testing in the Care of Lung Cancer Patients LV Sequist, VA Joshi, PA Janne, DW Bell, P Fidias - Clinical Cancer Research, 2006 - AACR ... 1 minute for 30 cycles; and 72°C for 10 minutes. PCR products are purified with AMPure magnetic beads (Agencourt, Beverly, MA). ...
• Funnybase: A Systems Level Functional Annotation Of Fundulus Ests For The Analysis Of Gene Expression JE Paschall, MF Oleksiak, JD VanWye, JL Roach, JA - feedback, 2006 - biomedcentral.com ... PCR products were purified manually in 96 well format using Sephadex ... 0.2 microfilter (Millipore, Billerica, USA) or robotically using AmPure (Agencourt, Beverly ...
• Bacteria In Oral Secretions Of An Endophytic Insect Inhibit Antagonistic Fungi F Password, E Entomology - Ecological Entomology, 2006 - Blackwell Synergy ... 2004. PCR products were purified using AMPure magnetic beads (Agencourt Bioscience Corp., Beverly, Massachusetts). Bacterial isolates ...
• Phylogenetics of the florally diverse Andean clade Iochrominae (Solanaceae) SDW Smith, DA Baum - American Journal of Botany, 2006 - Botanical Soc America ... min. All PCR products were purified with AMPure using manufacturer's protocols (Agencourt Bioscience, Beverly, Massachusetts, USA). ...
• Phylogenetic Relationships Of South American Lizards Of The Genus Stenocercus (Squamata: Iguania): A New Approach Using A General Mixture Model For Gene Sequence Data O Torres-Carvajal, JA Schulte, JE Cadle - Molecular Phylogenetics and Evolution, 2006 - puce.edu.ec ... were puriWed with either spin puriWca- tion columns (Qiagen, Inc.) or AMPure magnetic beads ... PCR and sequencing primers used in this study are listed in Table 2 ...
• Phylogeny And Biogeography Of Croton Alabamensis (Euphorbiaceae), A Rare Shrub From Texas And Alabama, Using DNA Sequence And AFLP Data BW VAN EE, N JELINSKI, PE BERRY, AL HIPP - Molecular Ecology, 2006 - Blackwell Synergy ... Polymerase chain reaction (PCR) products were cleaned using the AMPure magnetic bead method and sequenced at the University of Wisconsin Biotechnology Center ...
• RFLP Analysis Of The Mtdna Control Region In White Shrimp (Litopenaeus vannamei) populations from the eastern Pacific R Valles-Jimenez, PM Gaffney, R Perez-Enriquez - Marine Biology, 2006 - Springer ... The PCR prod- ucts were cleaned with a magnetic purification kit (AMPure, Agencourt Bioscience Corporation) following the manufacturer's protocols. ...
• Germline Gain-Of-Function Mutations In SOS1 Cause Noonan Syndrome AE Roberts, T Araki, KD Swanson, KT Montgomery, TA - Nature Genetics, 2006 - nature.com ... PCR products were purified using AMPure beads (Agencourt) and then sequenced using ABI BigDye 3.1 dye terminator chemistry and a 3730XL DNA analyzer.
• Basilar-Type Migraine Clinical, Epidemiologic, And Genetic Features M Kirchmann, LL Thomsen, J Olesen - Neurology, 2006 - AAN Enterprises ... Tetrad. PCR products were verified for correct length on agarose gel before being purified using AMPure from Agencourt...
• Atypical Haemolytic Uraemic Syndrome Associated with a Hybrid Complement Gene JP Venables, L Strain, D Routledge, D Bourn, HM ... - PLoS Med, 2006 - medicine.plosjournals.org ... PCR products were purified using magnetic microparticles (AMPure, Beckman) to remove unincorporated dNTPs, primers, and salts. Sequencing ...
• On The Wegener Granulomatosis Associated Region On Chromosome 6 P 21. 3 P Szyld, P Jagiello, E Csernok, WL Gross, JT ... - BMC Medical Genetics, 2006 - biomedcentral.com ... PCR conditions were: 1 x PCR buffer (Qiagen), 0.2 mM each dNTP, 0.2 pmol ... for 10 min. The product was purified with magnetic beads system (AMPure...
• Characterization of Gut-Associated Bacteria in Larvae and Adults of the Southern Pine Beetle, Dendroctonus frontalis Zimmermann A Vasanthakumar, I Delalibera, J Handelsman, KD ... - Environmental Entomology - bioone.org ... final extension at 72°C for 5 min. PCR products were purified using AMPure magnetic beads (Agencourt Bioscience, Beverly, MA). ...
• Molecular Phylogeography Of The Fruit Bat Genus Melonycteris In Northern Melanesia JN Pulvers, DJ Colgan - Blackwell Synergy ... COI PCR products were purified manually using AMPURE magnetic beads (Agencourt, Beverly, MA, USA). Generally, products were sequenced in both directions. ...
• Protist Genetic Diversity in the Acidic Hydrothermal Environments of Lassen Volcanic National Park, USA PB BROWN, GV WOLFE - Journal of Eukaryotic Microbiology, 2006 - Blackwell Synergy ... PCR primers were removed from PCR-amplified SSU rRNA genes with AMPure magnetic beads (Agencourt Biosciences, Beverly, MA) following the manufacturer's ...
• Early Detection And Progression Of Decay In L-Joints And Lap-Joints In A Moderate Decay Hazard Zone CA Clausen, TL Highley, DL Lindner - FOREST PRODUCTS JOURNAL - fpl.fs.fed.us ... minutes. The PCR products were cleaned using AmPure magnetic beads (Agencourt) following manufacturer's direc tions. Sequencing ...
• Evolutionary History Of Wolbachia Infections In The Fire Ant Solenopsis Invicta ME Ahrens, D Shoemaker - feedback, 2006 - biomedcentral.com ... Wsp PCR amplicons were purified for sequencing using AMPure magnetic beads (Agencourt Bioscience Corp.) and subsequently used directly in standard fluorescent ...
• High-Throughput Species Identification: From DNA Isolation To Bioinformatics DE RICHARDSON, JD VANWYE, A EXUM, RK COWEN, DL ... - Blackwell Synergy ... PCR products were then purified using the standard AmPure kit (Agencourt) protocol on the liquid-handling robot, but with a 1:1 ratio of magnetic beads to PCR ...
• Aberrant Promoter Methylation And Silencing Of The POU2F3 Gene In Cervical Cancer Z Zhang, PC Huettner, L Nguyen, M Bidder, MC Funk, ... - Oncogene, 2006 - nature.com ... The PCR conditions were: 95°C for 3 min, 94°C for 30 s ... Amplified products were purified using AMPure (Agencourt Bioscience Corp., Beverly, MA, USA), and were ...
• Preliminary Assessment Of Population Structure In The Mackerel Icefish (Champsocephalus gunnari) KL Kuhn, PM Gaffney - Polar Biology, 2006 - Springer ... PCR products were then 928 Page 3. purified for sequencing using AMPure beads according to manufacturer's protocol (Agencourt Bioscience, Beverly, MA). ...
• De Novo Gene Conversion In The RCA Gene Cluster (1q32) Causes Mutations In Complement Factor H Associated With Atypical Hemolytic Uremic Syndrome S Heinen, P Sanchez-Corral, MS Jackson, L Strain, ... - Hum Mutat, 2006 - doi.wiley.com ... PCR products were purified using magnetic microparticles (AMPure, Agencourt Biosciences) to remove unicorporated dNTPs, primers and salts. ...
• Strategies For Evaluating B*18 Allelic Diversity By Sequence-Based Typing Applied To Studies Of A Population From Singapore And African-Americans L Hou, B Tu, G Ling, T Tang, K Cao, NK Steiner, A ... - Tissue Antigens, 2006 - ingentaconnect.com ... approximately 1100 bp amplicon using AMPure according to the manufac- turer's instructions (Agencourt, Beverly, MA), direct sequencing of the PCR product was ...
• Genetic Testing In Atypical HUS And The Role Of Membrane Cofactor Protein (MCP; CD46) And Factor I THJ Goodship, V Fremeaux-Bacchi, JP Atkinson - 2005 - Springer ... 1-3]. PCR products are purified using magnetic microparticles (AMPure, Agencourt Biosciences) to remove unincorporated dNTPs, primers and salts. ...
• Fine Mapping And Evaluation Of Candidate Genes For Cervical Cancer On 11 Q 23 Z Zhang, DS Gerhard, L Nguyen, J Li, A Traugott, - Genes Chromosomes and Cancer, 2005 - doi.wiley.com ... For direct sequencing (POU2F3 and TRIM29), we separated PCR products by agarose gel electro- phoresis and purified them with AMPure (Agencourt, Beverly, MA). ...
• Conversion Analysis for Mutation Detection in MLH1 and MSH2 in Patients With Colorectal Cancer G Casey, NM Lindor, N Papadopoulos, SN Thibodeau, - 2005 - Am Med Assoc ... Sequencing of cDNA Products. The PCR fragments were purified using the AMPure purification system (Agencourt, Beverly, Mass). Sequencing ...
• Evolution And Diversification Of A Sexually Dimorphic Luminescent System In Ponyfishes (Teleostei: Leiognathidae), Including Diagnoses For Two New Genera JS Sparks, PV Dunlap, WL Smith - Cladistics, 2005 - Blackwell Synergy ... stranded amplification products were either desalted and concentrated using Qiagen Quick-Spin PCR Purification Columns, using AMPure (Agencourt Biosciences ...
• b-Catenin (CTNNB1) Gene Amplification: A New Mechanism of Protein Overexpression in Cancer G Suriano, N Vrcelj, J Senz, P Ferreira, H Masoudi - GENES, CHROMOSOMES & CANCER, 2005 - doi.wiley.com ... PCR products were purified with AmPure mag- netic beads (AgencourtBioscience, Beverly, MA), and a 2-ml aliquot of purified PCR product was then cycle-sequenced ...
• Association of NEDD4L Ubiquitin Ligase With Essential Hypertension CJ Russo, E Melista, J Cui, AL DeStefano, GL ... - 2005 - Am Heart Assoc ... DNA sequencing was performed by polymerase chain reaction (PCR) amplification of the target exon, purification of the product (AMPure solid-phase reversible ...
• Molecular Phylogenetics And Patterns Of Floral Evolution In The Ericales J Schoenenberger, AA Anderberg, KJ Sytsma - International Journal of Plant Sciences, 2005 - journals.uchicago.edu ... (1998). We cleaned PCR products using AMPure magnetic particles (Agencourt Bioscience, Beverly, MA)...
• Analyzing Ligation Mixtures Using A PCR Based Method PK Chandra, SK Wikel - Biol. Proced. Online, 2005 - biologicalprocedures.com ... 1). The PCR products were purified using the AMPure® solid-phase paramagnetic beads (Agencourt Biosciences, Beverly, MA, USA) and used for TA cloning into pCR ...
• A Deletion Causing Spontaneous Fracture Identified From A Candidate Region Of Mouse Chromosome 14 Y Jiao, X Li, WG Beamer, J Yan, Y Tong, D ... - Mammalian Genome, 2005 - Springer ... To verify the mutated gene (LGO) and the dele- tion, PCR products from both genomic and cDNA were purified using an AMPure PCR purification kit (Agencourt ...
• P53-Independent NOXA Induction Overcomes Apoptotic Resistance Of Malignant Melanomas JZ Qin, L Stennett, P Bacon, B Bodner, MJC Hendrix - Molecular Cancer Therapeutics, 2004 - AACR ... was amplified and PCR reactions were purified after thermocycling using solid-phase reversible immobilization-based technology (AMPure, Agencourt Biosciences ...
• Abnormal Cerebellar Development And Axonal Decussation Due To Mutations In AHI1 In Joubert Syndrome RJ Ferland, W Eyaid, RV Collura, LD Tully, RS Hill - Nature Genetics, 2004 - nature.com ... and purified them using either the PSI Clone PCR 96 kit (Princeton Separations) or the AMPure kit (Agencourt) in accordance with the manufacturer's protocols...
• Novel Mutations, No Detectable Mrna And Familial Genetic Analysis Of The Wiskott-Aldrich Syndrome Protein Gene In Six Japanese Patients With Wiskott-Aldrich Syndrome Y Sasahara, S Kawai, S Kumaki, Y Ohashi, M - European Journal of Pediatrics, 2000 - Springer ... to direct sequencing analysis with the same primers used for the PCR amplifcation ...The IgG fraction was obtained with Protein A column AMPure PA kit...
• Generation of Polymeric Immunoglobulin Receptor-Deficient Mouse with Marked Reduction of Secretory IgA S Shimada, M Kawaguchi-Miyashita, A Kushiro, T - The Journal of Immunology, 1999 - Am Assoc Immnol ... Screening was conducted by PCR using the following primers: sense, 5'-ATCTCGTCGTGACCCATGGCGATGC ... serum was recovered and IgG fraction purified with AMPure ...
• Reproducibility of Gene Expression Signature-Based Predictions in Replicate Experiments K Anderson, KR Hess, M Kapoor, S Tirrell, J - 1539 - AACR ... fragmentation buffer. The robotic cDNA and cRNA purification used AMPure PCR purification System (Agencourt, Beverly, MA). Fragmented cRNA ...
• Germline E-Cadherin Mutations In Hereditary Diffuse Gastric Cancer: Assessment Of 42 New Families And Review Of Genetic Screening Criteria SG Seruca, C Caldas, D Huntsman, SN Gilpin, D - British Medical Journal - jmg.bmj.com ... The remaining 17 ml of PCR product was purified using AmPure magnetic beads (Agencourt Bioscience, Beverly, MA)...
• Southern Hospitality: A Latitudinal Gradient In Gene Flow In The Marine Environment F Password - Blackwell Synergy ... PCR products were cleaned using either 96-well filter plates (Millipore Corp., Billerica, MA) or AmPure beads (Agencourt Corp. Beverly, MA)...
• Phylogeographic Structure Of The Fire Ant Solenopsis Invicta In Its Native South American Range: Roles Of Natural Barriers And Habitat Connectivity ME Ahrens, KG Ross, DDW Shoemaker - Evolution - bioone.org ... DNA amplicons were purified using Agencourt (Beverly, MA) AMPure magnetic beads and used directly in standard fluorescent cycle-sequencing PCR reactions
• DNA barcoding using chitons (genus Mopalia) ME Notes - Blackwell Synergy ... PCR products were cleaned using either 96-well filter plates (Millipore Corp.) or AMPure beads (Agencourt Corp., protocols available from manufacturer). ...
• The BARD1 Cys557Ser Variant and Breast Cancer Risk in Iceland SN Stacey, P Sulem, OT Johannsson, A Helgason, J - medicine.plosjournals.org ... PCR products were verified for correct length by agarose gel electrophoresis and purified using AMPure (Agencourt Bioscience; http:/ / www.agencourt.com). ...