Agencourt Ampure

Used by many top genomic centers, the Agencourt AMPure system is a highly efficient, easily automated PCR^† purification system that delivers high quality DNA. Requiring no centrifugation or filtration, Agencourt AMPure purifies both small and large PCR extension products in 96- or 384-well formats.

Application: PCR purification

Downstream Application Techniques: Genotyping applications (SNP, fragment analysis), clinical sequencing, sequencing, resequencing

The Agencourt AMPure XP system was developed to replace the current Agencourt AMPure offering. As such, the Agencourt AMPure product line will be discontinued in 2010. Read the obsolescence letter.

Agencourt AMPure Features

Efficient removal of unincorporated dNTPs, primers and salts
High-quality amplicon purification
No centrifugation or filtration
Manual or fully automated formats
Clean up of a variety of enzymatic reactions including PCR, rolling circle amplification and cDNA labeling

Process Overview

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

Effective Cleanup

The Agencourt AMPure system removes unincorporated primers, dNTPs, DNA polymerases and salts used during PCR amplification that can interfere with downstream applications. Agencourt AMPure routinely produces recovery rates greater than 90% for amplicons larger than 200 bp and greater than 85% for smaller PCR amplicons, such as the 165 bp fragment shown in Figures 1 and 2. Samples purified using Agencourt AMPure are ideal sequencing templates and routinely produce Phred 20 values of greater than 650 bases.

AMpure Clean Up

Figure 1: Shown are amplified pGEM* plasmid fragments of 165, 321, 410, 508 and 967 bp. Half of the reactions were purified using the Agencourt AMPure kit, and the percent recoveries calculated using a PicoGreen* assay. Samples were run on a 4% agarose gel. Lane MW: 100bp ladder; Lanes 1, 3, 5, 7 and 9: unpurified PCR products; Lanes 2, 4, 6, 8 and 10: Agencourt AMPure-purified samples.

Amplicon Length

Figure 2: Fragments sizes of 165, 321, 410, 508 and 967 bp from the M13 phage were amplified using PCR, purified using the Agencourt AMPure kit and the percent recovery calculated.

Scalable and Automation-Compatible

The Agencourt AMPure protocol does not require precipitation, filtration or centrifugation. The Agencourt SPRI technology is easily scaled and automation friendly, allowing both high throughput and format flexibility. Agencourt Bioscience has written and optimized software methods for Beckman Coulter's Biomek Laboratory Automation Workstations.

Ordering Information

To request a quote or make an inquiry use the on-line contact form.
In the United States and Canada call +1 800 361 7780.
Outside the United States and Canada consult the International Distributors list.

Product	Size	Kit Components	Product #
Agencourt AMPure 60 mL Kit	1,333/3,333 preps¹	Agencourt AMPure Reagent	A29152
Agencourt AMPure 450 mL Kit	10,000/25,000 preps¹	Agencourt AMPure Reagent	A29153

Related Products			Product #
Agencourt SPRIPlate 96R - Ring Magnet Plate			A29164
Agencourt SPRIPlate 384 Magnet Plate			A29165

1 25 µL/10 µL PCR reaction volume

† The PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffman-La Roche, Ltd.
* All trademarks are property of their respective owners.
Beckman Coulter, the stylized logo, Agencourt, AMPure, Biomek, CleanSEQ, Genfind, SPRI, and SPRIPlate are registered trademarks of Beckman Coulter, Inc.