Sample Submission Guidelines — Takeley
For QuickLane DNA Sequencing Projects shipping to Europe.
Recommendations for preparation of the material:
| Sample | Preparation | Quantity |
|---|---|---|
| Primers | 18 to 25 bases length, crude or cartridge purified 50°C < Tm < 75°C GC % : 40 to 60 |
Minimum volume of 10 µl at 5 µM concentration (up to 8 reactions). We do not adjust primer concentration. |
| Purified PCR products | Check amplified PCR products on agarose gel for the presence of a single band. | A minimum volume of 15 µl (up to 3 sequence reactions) and 5 µl per each additional sequence reaction - for PCR fragments < 1000pb : 15 ng/µl - for PCR fragments >1000 and <2000 : 25ng/µl - for PCR fragments >2000 and <3000 : 35 ng/µl |
| Non purified PCR products | Check amplified PCR products on agarose gel for the presence of a single band. Note: for your PCR pruification, the PUR add-on must be checked during the order. By default, PCR are not purified. |
A minimum volume of 15 µl (up to 3 sequence reactions) and 5 µl per each additional sequence reaction - for PCR fragments < 1000pb : 30 ng/µl - for PCR fragments >1000 and <2000 : 60ng/µl - for PCR fragments >2000 and <3000 : 90 ng/µl |
| Plasmids | Alkaline lysis + purification on membrane/column | Minimum volume of 15µl at 100 ng/µl concentration (up to 3 sequence reactions). Add 5µl for each addtional reaction. Please avoid TE. |
| BAC | Alkaline lysis + purification on membrane/column | Minimum volume of 15µl at 100 ng/µl concentration (for one reaction). Please avoid TE. |
| Single confinements | Isolated colony in agar stab or glycerol stock in tube, alternatively a petri dish. Tubes and petri dishes must have a CapMarker. If multiple colonies are to be picked from a petri dish, each colony should be circled and marked with a CapMarker. |
|
| Clones in plates | Isolated colony per well in glycerol stock. No CapMarkers are required for plates |
Shipment:
Primers and template DNA should be sent at ambient temperature.
- Tubes : samples and primers must be sent in 1.5 ml Eppendorf tubes identified with CapMarkers provided by Beckman Coulter Genomics.
- Plates : primers should be sent in 1.5 ml Eppendorf tubes identified with CapMarkers provided by Beckman Coulter Genomics. DNA templates must be in full 96-wells plate.
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