Targeted Resequencing by Automated Target Capture

Beckman Coulter Genomics new automated, high-capacity target capture sequencing service, offers a robust automated solution which streamlines the sample preparation for targeted next generation sequencing. The combination of Agilent SureSelect* Target Enrichment technology with Beckman Coulter’s SPRIworks Fragment Library System for automated library construction and Biomek Laboratory Automation Workstation for liquid handling, provides high quality, reproducible samples ready for sequencing. Beckman Coulter Genomics automated target capture solution allows for whole exome capture or custom targeting of genomic regions of interest. Sequencing on the Illumina* GAII or HiSeq 2000 platforms provides accurate data with reduced project turnaround time resulting from workflow automation and flexible sample multiplexing options.

Benefits of Targeted Resequencing

Reduces sequencing, data storage and processing requirements
Enables more samples to be sequenced - increased throughput, decreased cost per sample
Enables discovery of functional variations responsible for a disease

Benefits of Automated Target Capture

Higher throughput and faster project turnaround times
Greater reproducibility of sequence data
Decrease sample processing time from weeks to days
Increase sample prep capacity to allow for high level multiplexing for small target regions (up to 48-plex capture = 768 samples / HiSeq run)
Minimized manual handling and cross contamination risk
Automated LIMS sample tracking and data management

Applications

Assess genetic variations responsible for a disease
Major disease areas:

Oncology
Cardiovascular disease
CNS diseases
Inflammation
Metabolic disorders
Rare Mendelian diseases

Population studies

Workflow

Samples

Sequencing can be carried out from supplied purified genomic DNA from a variety of different sources including cell lines, blood, tissue, FFPE and xenograft.

Library Construction

Illumina paired end library construction is carried out on Beckman Coulter’s SPRIworks Fragment Library System to prepare the DNA for sequencing.

Target Capture

Target Capture is performed using the Agilent SureSelect Target Enrichment system, with a range of options available, including:

Whole Exome – 50 Mb Whole Exome kit
Custom regions – design and ordering of baits targeting custom genome region of interest using the Agilent eArray design tool

Automated library construction and capture allows for high level multiplexing with options up to 48-plex, depending on size of capture region.

Sequencing

Sequencing is performed on the Illumina GAII or HiSeq 2000. Coverage is dependent on sample source, purity and heterogeneity with recommendations of:

Tumor samples - 60-100x coverage
Non-cancer samples - 40x coverage

Data Analysis

Data analysis is included in the service and includes:

Alignment to a reference sequence
On-target analysis
Reporting of small Indels
SNP summary report including variant coordinates, base pair changes, dbSNP and coding region information

Results are delivered via a secure FTP site or are shipped on a portable hard drive depending upon the size of the dataset.

Getting Started

Beckman Coulter Genomics project managers will work with you to ensure appropriate study design for your project. This design will accommodate your specific needs and include recommendations for sample preparation and data analysis options. Please contact Beckman Coulter Genomics to discuss your next project.

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