* All trademarks are property of their respective owners.
Beckman Coulter, the stylized logo, Agencourt, CleanSEQ and SPRI are registered trademarks of Beckman Coulter, Inc.
Full Length Insert Sequencing
Still the gold standard technique for decoding DNA, Sanger sequencing remains advantageous for a number of experimental needs. For constructs larger than 25 kb, such as BACs, fosmids and cosmids, Beckman Coulter Genomics offers a world-class shotgun sequencing service as a fast and cost effective sequencing solution. Finishing strategies that employ our Primer Walking pipeline can be added to Full Length Sequencing projects to achieve publication quality sequence coverage.
Library Construction
Beckman Coulter Genomics has unique and highly automated library construction strategies optimized for both a high quality outcome and cost savings that can be transferred to the customer.
- Customized protocols for high and/or low copy vectors
- Template purification using Solid Phase Reversible Immobilization (SPRI) reagents
Sequencing and Data Assembly
Experience has proven that coupling automated SPRI template purification with Agencourt CleanSEQ dye terminator removal from sequencing reactions facilitates the generation of long, high quality Sanger sequencing reads resulting in better assemblies.
- DNA sequencing reactions using BigDye* Terminator v3.1
- Dye terminator removal with Agencourt CleanSEQ kit
- Sequence delineation on an ABI PRISM* 3730xl
- Base calling and data compilation
- Assembly with Phred, PHRAP and parcel genome assembler ARACHNE
Finishing
Pairing Full Length Sequencing with Beckman Coulter Genomics strategies for Genome Finishing improves repeat resolution, contig ordering and gap closure in assemblies.
- Expert Primer Walking for single coverage or publication quality finishing
- Finishing utilizing Consed with proprietary LIMS-integrated finishing software
Results
Results are delivered via a secure FTP site. Data delivery is personally overseen by a member of the Beckman Coulter Genomics staff to ensure simple and timely results transfer. Compiled data is available in multiple file formats including:
- FASTA
- .AB1
- .SCF
- .QUAL
