ChIP-Seq

ChIP-Seq experiments pair the sophistication of chromatin immunoprecipitation (ChIP) with the power of Next Generation DNA sequencing to elucidate the complex circuitry of genetic regulatory networks, genetic pathways and epigenetic mechanisms in living cells. Beckman Coulter Genomics has partnered with Genpathway* to offer complete and expert solutions for ChIP-Seq projects.

ChIP-Seq Enables:

• Discovery of novel transcripts and transcription factor binding sites
• Identification of genes regulated by known transcription factors and co-regulators
• Analysis of epigenetic events
• Direct comparison of regulatory events in different cell states (i.e. normal v. disease)
• Investigation of drug effects and other stimuli on regulatory pathways

The ChIP-Seq Assay:

1 - ChIP
Chromatin immunoprecipitation with validated antibodies isolates regulatory proteins cross-linked to their DNA binding sites.

2 - Library Generation
DNA binding sites released from the target proteins are prepared for analysis by sequencing.

3 - Next Gen Sequencing
DNA fragments are decoded with the Illumina* Genome Analyzer IIx.

4 - Data Analysis
Analysis of DNA sequencing data results in a genome-wide profile of DNA binding sites and transcribed genes.

The union of Genpathway’s proprietary chromatin immunoprecipitation assays and ChIP-Seq analysis tools with Beckman Coulter Genomics DNA sequencing expertise results in a total protocol able to advance gene regulation and genetic pathway research.

ChIP Sequencing Results

ChIP-Seq results from MCF7 cells shows an increase in SRC3 binding at two locations in the TFF1 gene after estrogen treatment.

Additional information about the ChIP-Seq method can be found at www.genpathway.com.