DNA Sequencing

Next Generation Applications

• Targeted Resequencing by Automated Target Capture - Targeted resequencing of the whole exome or custom regions of interest. Automated library construction and target capture for enhanced sample to sample reproducibility and flexible multiplexing options.
• Whole Genome Sequencing - De novo genome sequencing, whole genome re-sequencing and targeted re-sequencing capabilities, available across the full range of major next generation sequencing platforms, for detecting SNPs, InDels and structural variations.
• Metagenomic Sequencing - Investigate microbial communities by sequencing the16S gene or sampled fragments of the whole genome or transcriptome.
• De novo Sequencing - Sequencing of uncharacterized genomes, known genomes where significant structural variation is expected and genomes with high plasticity. Options available include sequence assembly and gap closure.
• cDNA and Small RNA Sequencing - RNA sequencing solutions are available for de novo transcriptome sequencing, whole transcriptome re-sequencing, digital gene expression using sensitive tag counting of transcripts and small RNA discovery.
• ChIP-Seq - Combination of chromatin immunoprecipitation (ChIP) and next generation sequencing for research into gene regulatory networks, genetic pathways and epigenetic mechanisms in living cells.
• SNP Discovery and Resequencing - Ultra-deep amplicon or targeted genome re-sequencing ideal for SNP detection of rare variants or for homogenous tissue such as cancer cells. Data analysis is also available for annotation of SNP results.

Sanger Applications

• QuickLane Express Sequencing - High quality sequencing for individual research samples, using customer supplied primers or free universal primers. Results returned within 24 hours.
• Single Pass Sequencing - High-throughput sequencing of plasmids or PCR products in 96- or 384-well format. For maximum efficiency, samples can be submitted as glycerol stocks, transformed libraries, agar plated libraries or unpurified PCR samples.
• Primer Walking and Finishing - Complete primer walking service including primer design and synthesis, quality scoring and sequence assembly. Ideal for unknown sequences from long amplicons or large plasmid inserts, as well as finishing work for whole genome or shotgun sequencing projects.
• Full Length Insert Sequencing - Shotgun sequencing service for large constructs such as BACs, fosmids and cosmids. Utilizing automated library construction and SPRI template purification for long reads and better assemblies.
• SNP Discovery and Resequencing - For SNP detection we offer bi-directional DNA sequencing for high quality resolution of homopolymers, insertions, deletions and microsatellites. Data analysis is also available for annotation of SNP results.

Illumina GAIIx and HiSeq 2000
The Illumina GAIIx and HiSeq 2000 platforms utilize reversible terminator-based sequencing by synthesis chemistry to deliver the highest sequencing output and fastest data generation rate of the next generation technologies. Ideal for whole genome re-sequencing, targeted resequencing, de novo sequencing and transcriptome sequencing.

Roche 454 GS FLX Titanium
The 454 GS FLX utilizes massively parallel pyrosequencing of bead bound templates and chemiluminescent base calling to yield long read lengths. Ideal for de novo sequencing, metagenomics and transcriptome sequencing.

ABI 3730XL
The ABI 3730XL Sanger sequencing platform utilizes capillary electrophoresis to generate sequences with read lengths up to 1200 bp and pass rates over 90%. Ideal for single sample sequencing, primer walking, shotgun sequencing and SNP detection.