† The PCR process is covered by patents owned by Roche Molecular Systems, Inc.,
and F. Hoffman-La Roche, Ltd.
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Beckman Coulter and the stylized logo are registered trademarks of Beckman Coulter, Inc.
DNA Sequencing
Sanger and Next Generation DNA Sequencing Solutions
Beckman Coulter Genomics recognizes that the DNA sequencing requirements of any research project derive from a unique combination of the experimental goal and the technical design. Sanger sequencing combined with a complete portfolio of Next Generation DNA sequencing technologies enables Beckman Coulter Genomics to support DNA research requiring traditional, Next Generation and hybrid DNA sequencing solutions. Cross platform proficiencies allow the flexibility of experimental design required to meet the objectives of a wide range of projects, irrespective of scale.
Technology Platforms
Sanger Sequencing
Still the gold standard technique for decoding DNA, Sanger sequencing remains irreplaceable for a number of specific experimental needs.
- Phred 20 read lengths: > 700 bp
- Pass rates: > 90%
Data delivery is personally overseen by an assigned Beckman Coulter Genomics Project Manager. Compiled data are available in multiple file formats including: .SEQ (FASTA), .AB1, .SCF and .QUAL.
454 Life Sciences Genome Sequencer FLX (GS FLX) Titanium
The long reads of the 454 Life Sciences GS FLX Titanium pyrosequencing platform have established utility in a variety of scientific applications and are particularly valuable for de novo sequencing projects.
- Read lengths: Averaging 350 - 400 bp
- Throughput (reads): ~1 million reads per run
- NCBI Data Formats: SFF data accepted since 2005
- Phred equivalent quality scores
- Multiplexing capabilities for flexible project design
Applied Biosystems SOLiD
As a Certified Sequencing provider, Beckman Coulter Genomics’ experience with the SOLiD sequencing platform surpasses that of any other sequencing provider.
- Read lengths: 50 bp fragment, 25 bp and 35 bp paired
- Throughput (reads): > 160 million reads per slide, fragment
- NCBI Data Formats: SOLiD Native or SRF
- Robust di-base chemistry for accurate base-calling
- Color space analysis with AB proprietary software for high confidence SNP calls
Illumina Genome Analyzer IIx (GAIIx)
With the highest per day throughput of the new technologies and a simple template amplification process simplifying multi-sample runs the Illumina GAIIx platform is well-suited for many research projects. Beckman Coulter Genomics is a CSPro certified provider of Illumina sequencing.
- Read lengths: 36 bp, 50 bp or 75 bp for fragment or paired sequencing
- Throughput (reads): > 120 million reads per run, fragment
- NCBI Data Formats: Illumina Native or SRF
- Paired end and mate paired sequencing approaches
- Ability to run up to 8 samples per experiment in physically separated channels
